Journal: Experimental and Therapeutic Medicine

Article Title: LSKL peptide alleviates subarachnoid fibrosis and hydrocephalus by inhibiting TSP1-mediated TGF-β1 signaling activity following subarachnoid hemorrhage in rats

doi: 10.3892/etm.2016.3640

Figure Lengend Snippet: LSKL peptide inhibited TSP1-mediated TGF-β1 signaling activity following SAH. Quantitative analyses of (A) TSP1 and (B) total TGF-β1 and active TGF-β1 in the CSF on days 3–5 after SAH. (C) Ratio of active TGF-β1 to total TGF-β1 in the CSF on days 3–5 after SAH. (D) Representative western blot bands of p-Smad2/3 and (E) quantitative analyses of p-Smad2/3 expression on day 5 after SAH. Relative densities of each protein have been normalized against the sham group. Data are expressed as the mean ± standard error of the mean (n=10). *P<0.05 vs. the sham group; # P<0.05 vs. the SAH+PBS group. SAH, subarachnoid hemorrhage; CSF, cerebrospinal fluid; TSP1, thrombospondin-1; LSKL, leucine-serine-lysine-leucine; PBS, phosphate buffer solution.

Article Snippet: These CSF samples were mixed and divided into three equal shares for the detection of TSP1, activated TGF-β1 and total TGF-β1 using the respective ELISA kit (Wuhan Boster Biological Technology, Ltd., Wuhan, China).

Techniques: Activity Assay, Western Blot, Expressing

Journal: Frontiers in Oncology

Article Title: Integration analysis of microRNAs as potential biomarkers in early-stage lung adenocarcinoma: the diagnostic and therapeutic significance of miR-183-3p

doi: 10.3389/fonc.2024.1508715

Figure Lengend Snippet: SESN1 is a potential target of miR-183-3p with clinical significance. (A) The TargetScan analysis analysis revealed that miR-183-3p binds to the 3’UTR of SESN1. (B, C) Comparison of miR-183-3p expression in both paired and unpaired LUAD tissue compared to normal tissue. (Blue: Normal tissues, Red: Tumor samples). *** p < 0.01. (D) The association between SESN1 expression and diverse immune subtypes in LUAD (C1: wound healing, C2: IFN-γ dominant, C3: inflammatory, C4: lymphocyte depleted, C5: immunologically quiet, C6: TGF-β dominant). (E-G) The correlations between miR-183-3p expression and clinical features in LUAD: (E) overall survival; (F) disease-specific survival; (G) progress-free survival. (H-J) ROC curve of SESN1 for LUAD diagnosis, pathological stage and pathological T stage. (K-P) Correlation of SESN1 expression with pathologic stage, pathologic T stage, and smoking status, as well as its impact on survival in LUAD. ** p < 0.01, *** p < 0.01.

Article Snippet: The membranes were incubated with antibodies against β-Tubulin, Caspase-3, Bax, Bcl-2, SESN1, and GAPDH (Boster Biological Technology), followed by incubation with secondary antibodies (Meilunbio).

Techniques: Comparison, Expressing, Biomarker Discovery

Journal: Frontiers in Oncology

Article Title: Integration analysis of microRNAs as potential biomarkers in early-stage lung adenocarcinoma: the diagnostic and therapeutic significance of miR-183-3p

doi: 10.3389/fonc.2024.1508715

Figure Lengend Snippet: The identification of SESN1 as a target gene of miR-183-3p. (A) The luciferase activity was measured following the co-transfection of miR-183-3p mimic with the Vector, SESN1 WT, and SESN1 Mut plasmids. ns, no significance, * p < 0.05. (B) The SESN1 mRNA expression in A549 and H1975 cells with miR-183-3p mimic and inhibitor plasmid. * p < 0.05, ** p < 0.01. (C) The SESN1 protein expression in LUAD cells with miR-183-3p mimic and inhibitor was measured by Western blot. (D) Histograms depict the intensity of SESN1 protein expression in both A549 and H1975 cells. ns, no significance, * p < 0.05, ** p < 0.01, *** p < 0.01.

Article Snippet: The membranes were incubated with antibodies against β-Tubulin, Caspase-3, Bax, Bcl-2, SESN1, and GAPDH (Boster Biological Technology), followed by incubation with secondary antibodies (Meilunbio).

Techniques: Luciferase, Activity Assay, Cotransfection, Plasmid Preparation, Expressing, Western Blot

Journal: iScience

Article Title: Pollutants corrupt resilience pathways of aging in the nematode C . elegans

doi: 10.1016/j.isci.2022.105027

Figure Lengend Snippet: Super Aggregators are identified in the iHg-, silica NPs-, and age-specific aggregome

Article Snippet: Interestingly, experiments with HSP-6 and RPL-7, two proteostasis proteins, showed opposite effects on C . elegans lifespan in previous studies: reduction of hsp-6 reduces lifespan , whereas the reduction of rpl-7 increased the lifespan ( ).

Techniques: Expressing